THE INFLUENCE OF SOLCOSERYL ON POPULATION AND CELLULAR PARAMETERS OF HELa AND RD CULTURES
Yu. A. Magakian, Z. A. Karalyan, E. M. Karalova, L. O. Abroyan, L. H. Hakopyan, M. G. Gasparyan, N. G. Jaghacpanyan, Z. B. Semerjyan, Z. R. Ter-Pogossyan
Institute of Molecular Biology NAS RA, Yerevan;
e-mail: tigmag@sci.am
Changes of population and cellular parameters of HeLa and RD cultures after introducing of solcoseryl in culture medium were studied by methods of scanning
cytophotometry and cytomorphometry. Monolayer density, proliferation activity, the number of dead cells in a monolayer, the number of nucleoli in nuclei and distribution of
cells in the populations by this parameter, RNA and DNA masses in nuclei and nucleoli, total volumes and surface areas of the nuclei and nucleoli were determined. It has
been shown that solcoseryl differently affects the cultures both on population and on cellular levels of their organization. The results of multi-parametric analysis of the influence
of solseryl on the cultures allow considering it as a biologically active compound with the features typical for cell and cell population growth regulating factors.
Key words: cell cultures proliferation, solcoseryl, nuclei, nucleoli
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