GENETIC HETEROGENEITY OF HEAT SHOCK PROTEIN SYNTHESIS
AS A FACTOR DETERMINING THE RESISTANCE OF MAMMALIA TO STRESSORS
A. L. Pukhalsky,1 G. V. Shmarina,1 I. V. Kapustin,2 S. V. Stukalov,1
D. A. Pukhalskaya,1 V. A. Alioshkin 2
1 Research Centre for Medical Genetics RAMS
and 2 G. N. Gabrichevsky Institite of Epidemiology
and Microbiology, Moscow;
1 e-mail: osugariver@yahoo.com
The relationship between the levels of 70 kDa family heat shock protein (Hsp) synthesis and lymphocyte
sensitivity to stressors was investigated. Lymphocyte cultivation in mitogen deprived culture medium and (or)
the cell treatment with alkylating agents have been used as a stress challenge. On the model of two inbred murine
strains genetically contrasting by the sensitivity to alkylating agents we succeeded in demonstration that the
basic level of Hsp synthesis depends on genotype. The quantity Hsp mRNA, as well as the intracellular level of
the proteins were significantly higher in BALB/c than in C57BL/6 mice. The mice characterized by higher Hsp
levels demonstrated higher resistance to alkylating agent action. The induction of surplus amount of Hsp by heat
shock increased the cell resistance to the alkylating agent melphalan. Lymphocyte isolated from high Hsp producers,
BALB/c mice, were more resistant to apoptotic signals induced by mitogen deprivation.
Key words: heat shock proteins, Hsp70, lymphocytes, stress, inbred mice, alkylating agents
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