GROWTH MODULATING AND CYTOTOXIC EFFECTS OF THE PEPTIDE FROM HEMOLYMPH OF BLOW FLY CALLIPHORA VICINA (DIPTERA, CALLIPHORIDAE)
IN VITRO
V. A. Plekach,1 I. V. Kozhucharova,1 I. V. Artcibasheva,1
L. L. Alekseenko,1 S. I. Chernysh 2
1 Institute of Cytology RAS, St. Petersburg, and 2 Biological Institute of St. Petersburg State University;
e-mail: gmdmk@mail.cytsb.rssi.ru
Biological activity of alloferon (AF), peptide, consisting of 13 a. a. isolated from hemolymph of experimentally
infected blow fly Ñalliphora vicina was studied. AF in concentrations form 1·10-5 to 250 μg/ml was
added into the culture medium of the target cell lines K562, J-96, P388D1, Hep22a and 3T3B-SV40. First two
days the peptide in concentrations 1·10-5 and 1·10-3 μg/ml in most cases stimulated the cell proliferative activity
and suppressed the cell growth when applied in concentrations 10 and 100 μg/ml. Trend in growth modulating
effect was dependent on duration of AF treatment. The peptide did not expressed cytotoxic effect with the
exception of destruction of P388D1 cells that was registered after 96 h incubation in the medium initially contained
100 μg/ml AF. Simultaneously, cytotoxic and growth modulating effects of doxorubicin and cytosinarabinoside,
as well as hybrid molecules, AF — cytosinarabinoside (cytal) and AF — doxorubicin (doxal) have been
studied. Doxorubicin and cytosinarabinoside expressed greater growth inhibition effect compared to the hybrid
molecules and AF itself. The results obtained with mass cell cultures were supported by experiments where
P388D1 cells clonogenic capacity was tested. Besides, it has been demonstrated that AF rapidly penetrates into
cytoplasm of J-96 cells and concentrates mainly into and around the cell nuclei.
Key words: peptide, cell line, cytotoxicity, growth modulating effect
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