TELOMERE LENGTH OF CORD BLOOD CELLS CHROMOSOMES AS ADDITIONAL QUALITY
CHARACTERISTIC OF SAMPLE FOR TRANSPLANTATION
P. Yu. Novikova,1,2,* O. V. Supilnikova,1 S. Yu. Novikova,1 A. S. Chrupina,1
D. A. Ivolgin,1 N. V. Smirnova,1,2 V. M. Mikhelson,2 O. G. Khurtsilava,1
A. B. Smolyaninov 1
1 Stem Cell Bank Pokrovski and 2 Institute of Cytology RAS, St. Petersburg;
* e-mail: polina_novik@mail.ru
Telomeres are the ends of the chromosomes and represent repeated DNA sequence and protein complex.
Telomeres shorten with each cell division, which limits proliferative potential of cells. There is a great progress
in clinical application of telomere length now. Additional characteristic of stem cell proliferation activity estimated
by telomere length can be an informative indicator of transplant quality. In this work, we analyzed 14
cord blood samples by flow-FISH, ELISA and gematologycal analisator, and also the number of
CD34 + CD45dim cells. Average telomere length of leukocytes fraction CB cells was 20.4 ± 4.9 % respectively
the control cells 1301 (T-cell lymphoblastic leukemia).
Key words: telomeres, stem cell, cord blood, flow-FISH, cell line 1301, transplant quality
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