INTERACTION BETWEEN NON-HISTONE CHROMATIN PROTEIN HMGB1
AND LINKER HISTONE H1
A. V. Fonin,1, 2, * Olga V. Stepanenko,1 K. K. Turoverov,1 V. I. Vorobyev 1
1 Institute of Cytology RAS, St. Petersburg,
and 2 St. Petersburg State Polytechnical University;
* e-mail: alexfonin@gmail.com
The fundamental possibility of interaction between non-histone chromatin protein HMGB1 and linker histone
H1 was studied in the solutions with different ionic strength by intrinsic UV-fluorescence, far and near-UV
CD and spectrophotometry. The obtained data allow us to assume that the increase of histone H1 content in the
HMGB1 solutions in a low ionic strength is accompanied by the destruction of HMGB1 associates. The interaction
between proteins of HMGB1 and H1 causes the increase in the number of ordered regions in the protein molecules
and the minor changes in their tertiary structure.
Key words: non-histone protein HMGB1, linker histone H1, chromatin, protein folding, intrinsically disordered protein
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