DETECTION AND IDENTIFICATION OF THE TUMOR-ASSOCIATED ANTIGENS
IN THE FRACTION ENRICHED WITH PLASMA MEMBRANES
OF THE ZAJDELA HEPATOMA CELLS
N. P. Teryukova,1,* O. A. Mirgorodskaya,2 M. V. Serebryakova,3 V. A. Ivanov 1
1 Institute of Cytology RAS, 2 Institute of Analytical Instrumentation RAS, St. Petersburg,
and 3 Scientific Research Institute of Physical-Chemical Medicine, Moscow;
* e-mail: npter@yandex.ru
Tumor-associated antigens 45, 57, 80 and 130 kDa were detected using tumor-specific rabbit immune serum
in the fraction enriched with plasma membrane of the rat Zajdela hepatoma cells and isolated on the immunosorbent.
Revealed proteins were identified as integrin β-1, ecto-nucleotide pyrophosphatase/phosphodiesterase-
3 (E-NPP3), basigin, epithelial cell adhesion molecule (EpCAM), α-fetoprotein (AFP) and protein chaperones
— glucose-regulated protein 78 (GRP78) and protein disulfide-isomerase (PDI) A1 by mass spectrometry
technique. Functions and characteristics of these proteins in tumor cells and some aspects of the Zajdela hepatoma
cells origin are discussed.
Key words: hepatoma, mass spectrometry, tumor-associated antigens, tumor-initiating cells, epithelial
cell adhesion molecule, epithelial-mesenchymal transition
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