CHARACTERISTIC OF TUMORS DEVELOPED AFTER TRANSPLANTATION
OF TRANSGENIC GFP-POSITIVE C57BL/6 MICE BONE-MARROW MESENCHYMAL
STEM SELLS TO mdx MICE MUSCLE
V. M. Mikhailov,1 E. V. Kaminskaja,1 V. B. Popov,1 S. N. Kuzovatov,1 N. S. Skripkina,2
G. B. Kosjakova,3 A. M. Zaichik,4 T. M. Grinchuk,1 N. N. Nikolsky 1
1 Institute of Cytology RAS, St. Petersburg, 2 St. Petersburg’s Pathologic-Anatomical Bureau,
3 Research Institute of Agree-Cultural Animals Genetics, and 4 Postgraduate Medical Academy, St. Petersburg;
e-mail: vmikhailov@mail.cytspb.rssi.ru
The purpose of the study was the morphological and histochemical characteristies of differentiation of tumors
developed after transplantation of GFP-positive mesenchymal bone-marrow stem cells (MSC) of transgenic
mice C57BL/6 into M. quadriceps femoris of mdx mice. The tumors occurred only after transplantation of
MSCs of 43—45th passages and did not arise after transplantation of MSCs of the 15th passage. No tumors developed
also after transplantation of MSCs of 43—45th passages into muscle of C57BL/6 mice. The average
weight of tumors appeared in 4 mdx mice studied was 1.3 ± 0.5 g. All four tumors were classified as mesenchymomas
because they originated from mesenchymal stem cells. Most of the periphery of the tumors was classified
as fibrosarcomas with mitotic index 0.9 ± 0.1 %. The central parts of tumors had areas with epithelial like
morphology of cells. Such cells showed positive reactivity for alcyan blue staining at pH 2.5, which indicated
chondrocyte nature of the cells. No mitosis was observed in epithelial like cells. In the tumors, there were also
areas with bone trabeculae containing megacaryocytes and foci of myeloid and erythrocyte hematopoiesis. There
were also areas with neuronal and glial cells, and accumulations of adipocytes. One of the tumors was classified
as a round cells sarcoma. The observed types of tumor cell differentiation in vivo were in accordance with
described in literature types of MSCs differentiation after induction in vitro with special inductors. The spectrum
of in vivo differentiation of transgenic GFP-positive MSCs after transplantation to mdx mice was broader
than the spectrum of in vivo differentiation of transfected or transformed in vitro adult MSCs after transplantation
to immunodeficient mice and mdx mice.
Key words: GFP-positive mesenchymal stem cells, transplantation, mdx mice, tumors, differentiation
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