NUCLEAR LOCALIZATION SEQUENCE OF BOVINE GAMMA-INTERFERON
PROVIDES TRANSLOCATION OF RECOMBINANT PROTEIN TO YEAST
PICHIA PASTORIS CELL NUCLEUS
A. E. Gradoboeva, M. V. Padkina
Department of Genetics and Selection, St. Petersburg State University;
e-mail: agrad74@mail.ru
It is known that heterologous protein production in microorganisms has negative influence upon a host cell.
In this regard, of particular interest is to know the fate of recombinant proteins in a cell. In this study, it was
shown that NLS of bovine gamma-interferon functions in yeast Pichia pastoris cells. The absence of the C-terminal
NLS of bovine gamma-interferon leads to cytoplasmic localization of the recombinant protein.
Key words: recombinant proteins, bovine gamma-interferon, nuclear localization sequence
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