THE CULTIVATION OF CELLS ON THE POROUS TITANIUM IMPLANTS
WITH THE DIFFERENT STRUCTURE
M. I. Blinova,1 N. M. Yudintzeva,1 N. S. Nikolaenko,1 I. L. Potokin,1
G. Raykhtsaum,2 M. Pitkin,2,3 G. P. Pinaev 1
1 Institute of Cytology RAS, St. Petersburg, Russia, 2 Poly-Orth International, Sharon, MA, USA,
and 3 Tufts University School of Medicine, Boston, MA, USA;
1 e-mail: mira.blinova@mail.ru
The data on human dermal fibroblasts and rabbit mesenchymal stromal cells cultivation on porous titanium
implants are presented in given paper. Two types of implants were used: type 1 — with irregular pores formed
by pressed titanium particles and type 2—with regular pores formed by coalescence of one-size titanium particles
into implant. The goal of this study was to choose the type of titanium implant porosity which ensures the
tightest interaction of titanium implant with surrounding tissue cells after implantation in the body. Cells were
cultivated on implants for 7 days and in both cases they formed confluent monolayer on the implants surfaces.
That indicated adhesion, migration and proliferation of cells on such implants. Condition of cells cultured on titanium
implants was controlled by scanning electron microscopy. The character of fibroblasts interaction with
given implants was different depending on porosity type of implants. On implants with irregular pores, the cells
were more spread and overlapped the pores spreading over neighbored particles. On implants with regular pores
that formed by one-size particles into implant, the fibroblasts covered these particles not overlapping the pores
and seldom interacted with neighbored particles by small outgrowths. There was no tight interaction of particles
into implant. In implants formed by pressed particles, the cells grew not only on the surface but also in the depth
of implant. Thereby, we suppose that more tight interaction of cells with titanium implant and, supposedly, tissues
with implant in an organism will take place in the case of implant structure formed by pressed titanium
particles.
Key words: titanium implant, fibroblasts, monolayer, topography, spreading, migration, adhesion
| Pdf |
Back |
Contents |
Main |
|
