SPONTANEOUS ANEUPLOIDY LEVEL IN BLOOD CELLS OF FERTILE FEMALES
N. V. Zotova,1 E. V. Markova,1 I. N. Lebedev,2 A. V. Svetlakov 1
1 Center for Reproductive Medicine, Krasnoyarsk, and
2 Research Institute of Medical Genetics, Tomsk Research Center RAMS;
e-mail: 1 krasivf@kcrm.ru;
2 igor.lebedev@medgenetics.ru
In this study we present the results of molecular-cytogenetic investigation of blood cells of fertile women of reproductive age. We used cultivated and
uncultivated cells and two sets of FISH probes with direct and indirect labeling. The mean aneuploidy levels of 4 chromosomes were determined and statistical
limits were scored. Aneuploidy levels determined in cultivated lymphocytes by multicolor FISH varied from 0.1 to 1.3 %. The mean aneuploidy level for all
chromosomes (13, 18, 21 and X) was 1.39 %. The limit mutation detection was 3.4 %. We found differences in results obtained with the use of direct and indirect
labeling. It was shown that cultivating process influenced the aneuploidy level. Absolute error of FISH technique was 0.13 %, relative error - 4.08 %.
Key words: spontaneous aneuploidy level, accuracy of FISH technique
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