NOVEL HUMAN EMBRYONIC STEM CELL LINES C612 AND C910
I. V. Kozhucharova,1 I. I. Fridlyanskaya,1 Z. V. Kovaleva,1 N. A. Pugovkina,1
L. L. Alekseenko,2 V. V.Zenin,1 K. M. Ivantsov,2 O. K. Leontieva,2 T. M. Grinchuk,1
N. N. Nikolsky 1, *
1 Institute of Cytology RAS and 2 International Centre of Reproductive Medicine, St. Petersburg;
* e-mail: Nikolsky@mail.cytspb.rssi.ru
Novel human embryonal stem cell lines C612 and C910 have been established from hatching blastocytes. Cells were cultivated in mTeST™ medium on mouse
fibroblast feeder-layers. They express common pluripotent markers such as alkaline phosphatase, Oct 3/4, SEEA-4, Nanog, Rex1. Immunophenotyping of these
cells by flow cytometry revealed expression of CD90 (Thy-1) and CD117 (c-kit) antigens and weak or no expression of CD13, CD34, CD45, CD130, HLA class I and
HLA class II antigens. This pattern of surface antigen expression is common for human embryonic stem cells. G-banding assay of C612 and C910 metaphase
plates showed that karyotypic structure of these cells was normal both in chromosome number and structure. The cells are pluripo-tent because of their capability
to generate embryoid bodies, undergo spontaneous differentiation and express markers of all germ layers: nestin, keratin, vimentin (ectoderm), á-fetoprotein
(entoderm), and muscle á-actinin (mesoderm). Thus, C612 and C910 cells have all attributes of typical human embryonic stem cells (diploid, capable of self-renewal,
express pluripotent markers and differentiate into three germ layers) and may be of potential use for fundamental and regenerative medicine researches.
Key words: human embryonic stem cells, cell lines, pluripotent markers, spontaneous differentiation, immunofluorescention
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