THE EXPRESSION OF CD25 IN PHYTOHEMAGGLUTININ- OR INTERLEUKIN-2-STIMULATED HUMAN PERIPHERAL BLOOD LYMPHOCYTES
V. V. Zenin, N. A. Aksenov, A. N. Shatrova, I. I. Marakhova 1
Institute of Cytology RAS, St. Petersburg;
1 e-mail: iim@mail.cytspb.rssi.ru
The timely expression of a high affinity receptor for interleukin-2 (IL-2R) in human peripheral blood lymphocytes
stimulated by various mitogens was examined by cytophotometric evaluation of the number of CD25
bearing cells (CD25+). In resting lymphocyte culture both phytohemagglutinin (PHA, 10 (μg/ml) and
12,13-phorbol dibutirate (PDBu, 10-8 M) and ionomycin (IM, 5·10-7 M) induce the long-lasting increase (during
48 h) in the number of CD25+ cells. Only in competent (not in resting) lymphocytes, pretreated by submitogenic
doses of PHA (1 μg/ml), interleukin-2 (IL-2) is capable to induce the time-dependent CD25 expression.
When comparing the time course of CD25+ expression and the blasttransformation it was found that the CD25
markers were revealed on small stimulated lymphocytes and all the large blasts were the CD+ cells with high-affinity
αβγc receptor for IL-2. In conclusion, the expression of α-subunit of IL-2R takes place during the IL-2-dependent
stage of T cell proliferation and may be directly induced by IL-2 via IL-2R.
Key words: human lymphocytes, interleukin-2, α-subunit, interleukin-2 receptor, CD25
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