FISH-ANALYSIS OF REGIONAL REPLICATION IN HOMOLOGOUS CHROMOSOMES IN HYBRID CELLS OBTAINED BY FUSION OF EMBRYONIC
STEM CELLS WITH SOMATIC CELLS
O. L. Podryadchikova, I. E. Pristyazhnyuk, N. M. Matveeva, O. L. Serov
Institute of Cytology and Genetics, Siberian Branch of RAS, Novosibirsk;
e-mail: serov@bionet.nsc.ru
The paper concerns FISH-analysis of regional replication of parental chromosomes 1, 3 and 6 in hybrid
cells obtained by fusion between Mus musculus embryonic stem cells (ESC) and M. caroli splenocytes. The data
demonstrated that parental chromosomes in the hybrid cells with near-diploid karyotype showed synchronous
replication in 70-75 % of tested cells that was comparable with diploid ESC and diploid fibroblasts. Synchronous
replication of parental chromosomes in hybrid cells with near-triploid karyotype was observed in
46-57 % of tested cells. However, it was correct in the case of hybrid cells with three copies of the tested chromosomes
whereas the ratio of synchronous replication in triploid cells with two copies was comparable or similar
to that in diploid cells. Hybrid cells with near-tetraploid karyotype showed high ratio of asynchronous replication
(over 50 %) comparable to those tetraploid ESC and tetraploid fibroblasts but significantly distinguished
from diploid cells. Thus, most hybrid cells with two copies of tested parental chromosomes showed their synchronous
replication. Unfortunately, the FISH-analysis is poor informative when it is used for studying cells
with more than two copies of tested chromosomes.
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