EFFECT OF FUCOIDAN ON THE ULTRASTRUCTURE OF MESOPHYLL CELLS OF DATURA STRAMONIUM L. AND ACCUMULATION OF
POTATO VIRUS X IN THEM
L. A. Lapshina, A. V. Reunov,1 V. P. Nagorskaya, T. N. Zviyaginseva, N. M. Shevchenko
Pacific Institute of Bioorganic Chemistry, the Far East Branch of RAS, Vladivostok;
1 e-mail: antreunov@mail.ru
Influence of fucoidan from brown alga Fucus evanescens C. Ag. on the development of infection induced by potato virus X (PVX) in Datura
stramonium leaves was studied. It as been shown that 24 h after the treatment of the leaves with fucoidan and following infection of them with PVX the
accumulation of virus particles in infected cells during early infection period was substantially less than that in untreated control. Using ultrastructure-morphometric
analysis, it has been established that fucoidan treatment increases at protein-synthesizing capability of cells (nucleolus dimension, amount of mitochondria and
rough endoplasmic reticulum membranes become increased). At the same time, the fucoidan treatment causes some activation of lytic compartment which leads
to destruction of virus particles and, therefore, might be considered as one of fucoidan-dependent protective mechanisms limiting virus accumulation in cells.
Fucoidan stimulation of the formation of PVX-specific laminated structures capable of virus particles binding is possibly another induced antiviral cell mechanism,
preventing from virus reproduction and transposition.
Key words: Datura stramonium, Fucus evanescens, fucoidan, potato virus X, mesophyll cells, infection
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