MOLECULAR GENETIC AND IMMUNOPHENOTYPIC ANALYSIS OF ANTIGEN PROFILING, OSTEOGENIC AND ADIPOGENIC POTENTIALS
OF MESENCHYMAL STROMAL CELLS FROM FETAL LIVER AND ADULT BONE MARROW IN RATS
M. N. Kozhevnikova,1 A. S. Mikaelyan, V. I. Starostin
Koltzov Institute of Developmental Biology, RAS, Moscow;
1 e-mail: mn_kozhevnikova@mail.ru
Comparative characteristics of mesenchymal stromal cells (MSC) from fetal liver and adult bone marrow are of great interest due to similar contribution
to organization of the hematopoietic microenvironments in these organs in different developmental periods. It is known that MSC play a pivotal role in organization
of hematopoietic stem cells "niches". Moreover, the histogenetic affinity between MSC from both sources is not ruled out. Antigen profiling using
immunocytochemistry and RT-PCR confirmed that cell populations under study corresponded to MSC criteria and had no hematopoietic, lymphoid and endothelial
cells after second passage. Comparative analysis of osteogenic and adipogenic marker expression revealed distinct differentiation potentials of MSC from two
hematopoietic organs: adipogenic potentials of hepatic MSC was weaker but ability to differentiate to osteogenic lineage was extremely low, if any, in comparison
with marked osteo- and adipogenic potentials of adult bone marrow MSC. Similar cell phenotype but unequal differentiation potentials under identical conditions
in vitro may be attributed to a distinct developmental program in pre- and postnatal histogenesis of MSC from different sources.
Key words: mesenchymal stromal cells, bone marrow, fetal liver, pre- and postnatal development, osteogenesis, adipogenesis
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