THE PATTERN OF CENTRIOLE IMMUNOSTAINING FOR TYROSINATED OR ACETYLATED α-TUBULIN CHANGES DURING MITOSIS IN 3T3 (A31)
CELLS BUT NOT IN SV40-3T3 CELLS
E. E. Balashova,1 P. G. Lokhov,2 V. B. Bystrevskaya 1
1 Cardiology Research Center and 2 Institute of Biomedical Chemistry RAMS, Moscow;
1 e-mail: balashlen@mail.ru
Comparative electron microscopic analysis of the patterns of centriolar and cytoplasmic microtubules immunostaining
for acetylated or tyrosinated α-tubulin was performed during the cell cycles of the mouse diploid
3T3 (A31) and virus-transformed heteroploid SV40-3O3 cell lines. It was shown that the pattern of centriole immunostaining
changed during mitosis in control 3T3 (A31) cells, but not in tumorigenic SV40-3T3 cells.
A sharp change in the pattern of centriole immunostaining was observed in those cell cycle periods when the
protein organization of centrosome underwent changes during interphase/mitosis or mitosis/interphase transitions
due to the rearrangement of microtubule system. A high level of centriole immunostaining for tyrosinated
and acetylated α-tubulin was observed at all cell cycle stages with the exception of entering mitosis for the
former and the end of mitosis for the latter.
Key words: mitosis, tubulin, centriole
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