INTERRELATION BETWEEN THE GLYCOGEN CONTENT IN HEPATOCYTES AND THEIR SIZE IN NORMAL AND CIRRHOTIC RAT LIVER
N. N. Bezborodkina, A. A. Vahtina, E. V. Baidyuk, G. S. Yakupova, B. N. Kudryavtsev
Institute of Cytology RAS, St. Petersburg;
e-mail: cellpath@mail.cytspb.rssi.ru
The dependence between the size of hepatocytes and glycogen content in them was investigated in normal and cirrhotic liver from fasting rats as well as
from rats in 10 and 60 minutes after per os glucose administration. The cytophotometric method used allows glycogen and DNA contents determining
in the same cell and its dry mass as well. It has been shown that the dry mass and the glycogen content in hepatocytes from normal and cirrhotic liver are
proportional to genes dose. Normal liver hepatocytes of various ploidy classes show distinct correlation between the cell size and the glycogen content.
No similar dependence is observed in hepatocytes population from the cirrhotic liver, apparently, because the liver lobule structure is disturbed, and heterogeneity
of the hepatocytes microenvironment condition is increased.
Key words: hepatocytes, refeeding, glycogen, ploidy, dry mass, cirrhotic liver
Back
Contents
Main
