INFLUENCE OF DROSOPHILA MELANOGASTER GENOTYPE ON BIOLOGICAL EFFECTS OF ENDOCYMBIONT WOLBACHIA
(STAMM wMelPop)
D. A. Voronin,1 A. M. Bocherikov,1, 2 E. M. Baricheva,1 I. K. Zakharov,1, 2
E. V. Kiseleva 1, *
1 Institute of Cytology and Genetics, Siberian Branch of RAS, Novosibirks, and 2 Novosibirks State University;
* e-mail: elka@bionet.nsc.ru
Comparative analyses of symbiotic bacteria Wolbachia (stamm wMelPop reducing lifespan of flies) morphology in normal and mutant strains of
Drosophila melanogaster as well as the influence of Wolbachia on the host cell ultrastructure have been done. Wolbachia infected
D. melanogaster mutation strains Trithorax-like - Trl362/TM3, Sb1 Ser y+ and Trlen82/TM3,
Sb1 Ser y+ have been received by special flies crossing. Uninfected strain D. melanogaster white-1118
(w1118) have been obtained by antibiotic treatment of initially infected strain D. melanogaster [w]w1118. Complex of different
methods and approaches let to investigate for the first time the morphology of cell structure before and after bacterial infection of insects and to value the bacterial
presence effect on flies viability and reproduction of normal and mutant flies. Morphology af cytoplasmic compartments in early embryos and eggs layed by infected
and uninfecyed females Trl362/TM3, Sb1 Ser y+ and Trlen82/TM3, Sb1 Ser y+
have been analyzed. Electron microscopy has shown that D. melanogaster embryos contain typical Wolbachia contacting with different host
organelles that verify preservation of their functional activity. Atificial mitochondria and Wolbachia (wMelPop) of unusual morphology with defective bacterial
membranes have been visualised in D. melanogaster [w]Trl362/TM3, Sb1 Ser y+. Wolbachia presence in
ovarium cells from strains [w]Trl362/TM3, Sb1 Ser y+ and [w]Trlen82/TM3, Sb1
Ser y+ did not influence on eggs quantity layed by females. We have demonstrated for the first time that lifespan of infected and uninfected strains:
D. melanogaster Trl362/TM3, Sb1 Ser y+ and Trlen82/TM3, Sb1 Ser y+
were similar. However the lifespan of imago from strain [w]w1118 was lower in comparison to those from strains Trl362/TM3,
Sb1 Ser y+ and Trlen82/TM3, Sb1 Ser y+. It suggests that either chromosomal balancer
TM3 or Trl mutation play an importance role in host-symbiotic relationship. Next experiments have revealed that lifespan of homozygotic flies decreased essentially
and was close to lifespan of strain [w]w1118. Data obtained confirm that chromosomal balancer TM3 can affect on symbiont-host relationship.
Key words: Wolbachia, Drosophila melanogaster, symbiotic bacteria, ultrastructure, symbiosis, electron microscopy, length of life
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