EFFECT OF LOW TEMPERATURE ON 3H-URIDINE INCORPORATION IN OOCYTES OF TILAPIA MOSSAMBIQUE
D. A. Chmilevsky
Biological Research Institute of St. Petersburg State University;
e-mail: ichtyol1943@mail.ru
Using 3H-uridine dynamics of RNA synthesis in oocytes of tilapia which grow at different temperatures was studied. Intact fishes were
cultivated at the temperature 25-26 °N, experimental ones - 19-21 °N. Before 3H-uridine injection, experimental fishes were cultivated at low
temperature for 5 days, then both intact and experimental fishes were injected by 3H-uridine and experiment was continued for 5 days. Under normal
temperature in 8 h after 3H-uridine injection, chromosomes of early previtellogenic oocytes were strongly labeled and RNA migrated to the cytoplasm
very fast. Nucleoli of early vitellogenic oocytes were intensively labeled as well. In 1, 2 and 5 days after injection, concentration of labeled RNA in cytoplasm of the
oocytes at all stages was increased. Oocytes of the experimental fishes showed low level of RNA synthesis. Probably, this connected with slow migration of
3H-uridine into gonads of fishes, low level RNA synthesis on chromosomes and nucleoli of oocytes and more low migration of labeled RNA from
nucleus to cytoplasm of the oocytes.
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