EFFICIENT METHOD OF ANALYSIS OF OPTICAL SPECTRA FROM KINETIC STUDIES
A. N. Skvortsov
Institute of Cytology RAS, St. Petersburg;
e-mail: skvor@mail.cytspb.rssi.ru
The application of principal components for the analysis of kinetic data obtained by optical spectroscopy is described. The use of singular value decomposition
(SVD) for stable and reproducible generation of principal components, details of realization, advantages and drawbacks of the method are discussed. The described
method with minor modifications may be used in a wide variety of UV-spectroscopy applications in molecular biology and biophysics. The developed method was
applied to study the reaction of platinum anticancer drug, cisplatin, with DNA and methionine. Use of sensitive UV-spectroscopy allowed to study low platinum
concentrations, typical for biological systems. It has been shown, that reactions of cisplatin with DNA and L-methionine generally follow the same pathway both at
high and low concentrations.
Key words: UV-spectroscopy, kinetic studies, chemometrics, principal component analysis, spectrum analysis, cisplatin, platinum drugs
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