THE SHIFT IN PLASMA MEMBRANE H+-ATPASE ACTIVITY IN COLEOPTILE CELLS WITHIN MAIZE SEEDLING DEVELOPMENT
E. L. Rudashevskaya,1 A. Yu. Yakovlev,2 O. V. Yakovleva,3 M. F. Shishova 4
1, 4 St. Petersburg State University, Department of Plant Physiology and Biochemistry,
and 1-3 V. L. Komarov Botanical Institute RAS, St. Petersburg;
4 e-mail: mshishova@mail.ru
The activity of plasma membrane H+-ATPase was cytochemically investigated in parenchyma cells of maize coleoptile. The intensity of ATP
hydrolysis was determined by deposition of electron density sediment formed by lead and inorganic phosphate. The shift in the enzyme distribution and its hydrolytic
activity in the plasma membrane of maize coleoptile cells were revealed within seedling development. The intensity of precipitation was quantified by Image
Pro® Plus. The maximum precipitation was determined in plasma membrane of coleoptile cells of 4-days old seedlings. The data obtained coincide with
the results of previous biochemical investigation of H+-ATPase hydrolytic activity, provided with purified plasma membrane vesicles. Nevertheless the
increase in quantity and(or) activity of the enzyme was observed at the 4th day of seedling development which was characterized by slowing down of
growth intensity. The results show the possibility of changes in quantity and distribution of the enzyme in plasma membrane during the differentiation process of cell
elongation.
Key words: H+-ATPase, plasma membrane, cell elongation, maize coleoptile
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