EFFECT OF ALPHA-LIPOIC ACID ON FIBROBLASTS 3T3 AND 3T3-SV40. COMPARISON WITH N-ACETYLCYSTEINE ACTION
E. A. Vakhromova, Y. S. Polozov, K. M. Kirpichnikova, N. D. Aksenov, I. A. Gamaley
Institute of Cytology RAS, St. Petersburg;
e-mail: igamaley@mail.cytspb.rssi.ru
In this study we investigated the effect of alpha-lipoic acid (ALA) in concentration range 0.7-5.0 mM on the intracellular level of reduced glutathione, the cell cycle
phase distribution, the structure of microfilaments and microtubules of normal (3T3) and transformed (3T3-SV40) fibroblasts. We obtained that ALA increased the
glutathione content in transformed cells, but did not change its level in normal cells, induced cell cycle arrest of 3T3 cells (but not 3T3-SV40 cells), and disrupted actin
microfilaments in cells of both lines. The effect of ALA was compared with N-acetylcysteine (NAC) action. The whole complex of findings allows us to affirm that each of
these antioxidants acts on its own target molecules in normal and transformed cells and activates different signal and metabolic pathways in these cells. But at the same
time the intermediate steps of ALA and NAC action can be common (alteration of the intracellular level of glutathione, reorganization of actin cytoskeleton, etc.).
Key words: alpha-lipoic acid, N-acetylcysteine, reduced glutathione, cell cycle, actin microfilaments, microtubules, fibroblasts
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