THE INFLUENCE OF DOPAMINE ON THE ULTRASTRUCTURE OF BHK-21 CELLS
V. S. Shubina,1,* M. B. Abramova,2 V. P. Lavrovskaya,2 L. L. Pavlik,2
E. I. Lezhnev,1, 2 D. A. Moshkov 1, 2
1 Pushchino State University, Moscow region, and
2 Institute of Theoretical and Experimental Biophysics RAS, Pushchino, Moscow region;
* e-mail: shubinavictoria@rambler.ru
The influence of dopamine on the haloperidol of BHK-21 cells being in suspension or attached to supstrate was investigated. It was shown that the ultrastructural
changes affected mainly the cellular loci enriched by the cytoskeleton actin such as intercellular desmosome-like contacts, microvilli and cortical layer or mesh just
beneath the plasmatic membrane. The desmosome-like contacts were hypertrophied, their electron density was increased and fibrilar bridges appeared in specialized
contacts. Many microvilli fused with each other and with plasma membrane of the neighboring cells, or, on the contrary, split up. Frequently, the membrane surface
between microvilli and particularly their apical parts was seen to be pierced by thin thread, morphologically similar to ac-tin filaments. The cytoplasmatic matrix onto
ultrathin sections had blotched appearance and at the ultrastructural level was represented by numerous randomly oriented actin filaments. The effect of dopamine was
more pronounced in the BHK-21 cells when being in suspension than in attached to the supstrate ones, which presumably occurred due to known lesser differentiation
of the cytoskeleton in the formers. Finally, it was established that the preliminary blockade of cellular D2 receptors with haloperidol neither affected the ultrastructure of
BHK-21 cells nor prevented the following effect of dopamine. The data obtained suggest the direct interactions of dopa-mine with the actin cytoskeleton.
Key words: BHK-21 cells, dopamine, haloperidol, cytoskeleton
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