CYTOLOGICAL, MORPHOLOGICAL AND FUNCTIONAL CHANGES OF RUSSIAN STURGEONS'S SPERM CELLS AFTER CRYOPRESERVATION
G. V. Zemkov, T. I. Akimochkina 1
Department of Biotechnologies and Bioecology of Astrakhan State University, Astrakhan;
1 e-mail: akimochkinatanja@mail.ru
The article presents some experimental results concerning the problem of genetic conservation of valuable and endangered animal species. Biotechnical
development of the cryopreservation of spermatic cells for the purpose of their extended storage at low and ultra low temperatures is a priority line of the investigations
in the topical area and implies creation of animal genomes cryobanks. It is known that water crystals of plasma and the cell itself often cause the spermatholysis in the
course of freezing. That is why the search for the substances with cryoprotective properties continues up to the present moment. In this study, we investigated cryoprotective
properties of glycerin, dimethyl sulfoxide and heparin in different proportions using spermatic fluid of sturgeon Acipenser guldenshtadti (Brandt). These results
obtained were compared with the cryoprotectors of well-known composition. In addition, freezing and storage of the spermatic fluid were executed in two variations: at low
and ultralow temperatures. Cold tolerance of the sperm cells was estimated by the sperm mobility and by light microscopy morphological analysis of the cells. It was
shown to be linked to the composition of cryoprotectors. The heaviest violations were observed under addition of osmotic active substances, some inorganic compounds
among them. Principle of our modification is based on that we used fluid substances: glycerin, dimethyl sulfoxide, heparin eliminating water as a solvent. The results
obtained showed that our modified cryoprotectors and the standard cryoprotectors had equal efficiency. The article also presents the data on the spermatic fluid freezing at
low temperature. They are important in case of modern freezing systems.
Key words: cryopreservation, cold tolerance, defrostation, sperm, cryoprotectors, Acipenser guldenshtadti (Brandt), cytomorphological analyse
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