UNTARGETED QUANTUM DOTS IN CONFOCAL MICROSCOPY OF LIVING CELLS
T. N. Belyaeva, A. V. Salova, E. A. Leontieva, T. P. Mozhenok, E. S. Kornilova, S. A. Krolenko
Institute of Cytology, RAS, St. Petersburg;
e-mail: tbel@mail.cytspb.rssi.ru
The problem of non-specific binding of quantum dots (QDs) with cells is very important but not fully understood taking into account the possible application of QDs in
medical and fundamental studies. The interactions of untargeted CdSe/ZnS QDs with isolated frog muscle fibres, HeLa cells and J774 cells were investigated. The
observations were performed on living cells using laser confocal microscopy (Leica TCS SL). The QDs covered with polyethylenglycol without any functional reactive groups
with emission maximum at 565 nm were used in the study. This type of QDs is suggested to prevent an interaction of QDs with biological molecules. It has been shown that
QDs do not enter HeLa cells, T-system and sarcoplasm of skeletal muscle fibres. However, during long-term incubation J774 cells can uptake QDs. The data obtained has
demonstrated the diversity of interactions of untargeted QDs with different cell types and are important for understanding of the problems of non-selective uptake and
cytotoxicity of QDs.
Key words: quantum dots, polyethylenglycol, confocal microscopy, muscle fibres, J774 cells, HeLa
| Pdf |
Back |
Contents |
Main |
