COMPARISON OF FIBROBLASTS-LIKE CELL DIFFERENTIATION CAPACITIES OF HUMAN BONE MARROW, ADIPOSE TISSUE, HAIR PAPILLA AND
DERMAL FIBROBLASTS
E. V. Kiseleva,1, 2 E. S. Chermnykh,1, * E. A. Vorotelyak,1 A. I. Volozhin,2
A. V. Vasiliev,1 V. V. Terskikh 1
1 N. K. Koltzov Institute of Developmental Biology RAS, Moscow, and 2 Moscow State University of Medicine and Denlistry;
* e-mail: elinachermnykh@mail.ru
We compared the morphology and differentiation characteristics of the human cells from bone marrow, adipose tissue, hair papilla and skin dermis. All cell types
showed fibroblastic morphology. Immimofluorcscent analysis showed that adipose tissue derived stem cells (ADAS) and hair papilla cells (HPC) expressed CD 105,
CD49d and STRO-1, bone marrow mesenchymal stem cells (BMSC) were absent for CD49, dermal fibroblasts (DFb) expressed CD49 and STRO-1 at low level.
Populations of ADAS, BMSC and HPC had similar capacities to lipid and bone differentiation. Following exposure to appropriate induction stimuli, these cells
changed phe-notype and expressed specific cell markers. However, the rate and extent of HPC differentiation were lower in comparison with populations of ADAS and
BMSC. We propose that all investigated cell populations contain primitive progenitor cells with mesenchymal stem cell properties.
Key words: bone marrow mesenchymal stem cells, adipose tissue derived stem cells, hair papilla cells, differentiation
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