2009. Vol. 51, N 1, p. 5-11
CHARACTERISTICS OF HUMAN LIPOASP1 RATE-ISOLATED MESENCHYMAL STROMAL CELLS CULTIVATED UNDER A LOWER OXYGEN TENSION

L. B. Buravkova, O. S. Grinakovskaya, E. R. Andreeva, A. P. Zhambalova, M. P. Kozionova

Institute for Biomedical Problems RAS, Moscow;
e-mail: grinakovskaya@imbp.ru

In the present study we investigated the effect of low oxygen concentration in the cultivation medium on proliferation, viability and immunophenotype of human mesenchymal stromal cells isolated from lipoaspirate (lMSC). It was shown that proliferation activity of the cells under hypoxic conditions was, on average, 2,9 times higher compared to that under commonly accepted - normoxic conditions. Redused oxygen level in the culture medium did not cause any change in lMSC viability and immunophenotype. Thus, permanent culture of lMSC in the medium with a lower oxygen tension can prove an efficient approach to obtain a higher mass of cells which maintain their characteristics in a shorter period of time, which can be of demand for regenerative medicine.

Key words:  mesenchymal stromal cells, lipoaspirate, hypoxia, notmoxia, proliferation, immunophenotype


Back    Contents    Main