THE STUDY OF PREIMPLANTATION DEVELOPMENT OF MICE EMBRYOS LABELLED WITH GREEN FLUORESCENT PROTEIN GENE (EGFP)
A. V. Sorokin,1 E. M. Nonyashvili,1 O. V. Kidgotko,1 L. K. Sasina,1, *
T. D. Aleynikova,1 M. M. Shavlovsky,1 S. I. Gorodetzky,2 A. P. Dyban 1
1 Institute of Experimental Medicine RAMS, St. Petersburg, and 2 Institute of Molecular Biology RAS, Moscow;
* e-mail: sassinal@googlemail.com
One of the crucial problems of developmental biology is the study of mechanisms of regulation of gene expression in early embryogenesis. Here we studied
dynamics of mosaic appearance of a marker fluorescent protein in vitro developing mice embryo derived from zygotes after microinjections to male pronuclei
of cloned DNA fragment carrying EGFP under control of different promoters. Main attention was paid to initial stages of development, when structural
rearrangements and reprogramming of both parental genomes, activation of zygotic genes, and control of development by embryo genome take place.
Key words: mice embryos, EGFP gene, transgenetic embryos, zygotic genes activation
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