DIFFERENTIATION OF THE STABLE HYALINE CELLS IN RESPONSE TO FOREIGN PARTICLES INJECTIONS INTO THE LARVAE OF BLOWFLY
CALLIPHORA VOMITORIA
T. V. Kind
Laboratory of Biopharmacology and Immunology of Insects, Biological Institute of St. Petersburg State University;
e-mail: tatiana.kind@mail.ru
Injection of foreign particles (charcoal and human erythrocytes) into the larvae of Calliphora vomitoria provokes the complex immune response including
their phagocytosis, nodulation and encapsulation by plasma-tocytes and thrombocytoids. Precursors of thrombocytoids and analogs of Drosophila lamellocytes
are very frequent during the periods of feeding and crop emptying, but fully disappear in wandering larvae. Injection of charcoal or erythrocytes into crop emptying
larvae leads also to a dramatic increase in the number of stable hyaline cells, precursors of thrombocytoids. The hyaline cells differentiate from prohaemocytes and,
quite possibly, from young weakly-specialized plasmatocytes in a day after injection. Later they are transformed to prothrom-bocytoids and thrombocytoids.
The number of hyaline cells and young plasmatocytes in the crop emptying larvae of C. vomitoria is far greater than that in the same age larvae of
C. vicina. Presumably it accounts for significantly increasing rate of stable hyaline cells differentiation in the injected larvae of C. vomitoria. Their part
after injection of charcoal particles or erythrocytes may reach 40-50 % of the main haemocyte number compared to 20-25 % in C. vicina. After completion
of the crop emptying, the rate of hyaline cells differentiation in response to the foreign particles injection is evidently reduced but remains to be distinctly visible.
Injections of saline also stimulate the differentiation of the stable hyaline cells from prohaemocytes but elevation of their amount is more weak and gradual. The
bacterial immunization and needle prick show no effect. The treatments, inducing the rising of hyaline cells differentiation, lead also to pupariation delay. This
correlation suggests involvement of the endocrine system into this process.
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