RETROPOSONS OF ALU FAMILY FROM CIS-REGULATORY MODULES OF DNAse II AND CAML GENES EFFECT GENE
EXPRESSION IN A549 AND HEK293 CELLS
N. M. Usmanova, V. I. Kazakov, N. V. Tomilin
Institute Cytology RAS, St. Petersburg;
e-mail: nmusmanova@gmail.com
Different fragments of promoters of deoxyribonuclease II (DNAse II) and calcium-modulating cyclophilin ligand (CAML) associated with Alu
family repeats have been inserted into a luciferase reporter vector. These constructions were introduced into A549 and HEK293 cell lines and after transient
transfection we lysed cells and analysed luciferase activities in these lysates. It has been shown that Alu repeats localized in constructions influence expression
of luciferase. Therefore, Alu copies which are associated with cis-regulatory modules of protein-coding genes have biological activity.
Key words: Alu repeats, gene expression, DNAse II, CAML
Back
Contents
Main
