THE CYTOCHROME Ñ OXIDASE ACTIVITY IN MITOCHONDRIA OF CARDIOMYOCYTES OF ISOLATED CARDIAC TISSUE UNDER LONG-TERM
HYPOXIC INCUBATION
V. B. Saprunova, I. M. Solodovnikova, L. E. Bakeeva 1
A. N. Belozersky Research of Physico-Chemical Biology, Institute Moscow State University;
1 e-mail: bakeeva@imec.msu.ru
Using method of electron microscopic histochemistry based upon the oxidative polymerization of 3,3'-diaminobenzidine (DAB) to reveal cytochrome ñ oxidase
activity we identified that long hypoxic incubation of isolated small pieces of cardiac tissue during 72 h caused changes in mitochondrial ultrastructure followed by a
breach of functional activities of mitochondria, and, in particular, complex IV of the respiratory chain. But for all that, small electron-dense mitochondria appearing
inside electron-light mitochondria ("mitochondria inside mitochondria") stained positively for cytochrome ñ oxidase activity along the full length of cristaes. The results
obtained are discussed in connection with conception of changes in the mitochondrial reticulum ultrastructure during mitoptosis.
Key words: mitochondria, cardiomyocytes, electron microscopy, intracellular regeneration, hypoxia, mitoptosis
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