REORGANIZATION OF ACTIN CYTOSKELETON IN 3T3-SV40 CELLS AND THEIR SENSITIVITY TO LYSIS BY NATURAL KILLER CELLS
N. A. Filatova, K. M. Kirpichnikova, I. A. Gamaley 1
Institute Cytology RAS, St. Petersburg;
1 e-mail: igamaley@mail.cytspb.rssi.ru
The present work was aimed to examine whether the actin reorganization of 3T3-SV40 cells influences their sensitivity to natural killer (NK) cells activity.
The effects of N-acetylcystein (NAC) and latrunculin B, actin depolimerizator, on both cellular parameters were studied. Experiments with NAC demonstrated
that 3T3-SV40 sensitivity to NK cells activity remained unchanged under the disordered microfilaments but decreased upon the appearance of structured
stress-fibres. The data on latrunculin B action resulted in the opposite conclusion: the more microfilaments disorganization in the presence of latrunculin B
the lesser 3T3-SV40 sensitivity to lysis by NK cells. These facts suggest that relations between microfilament integrity in 3T3-SV40 cells and their sensitivity
to NK cells are rather independent. The latter confirms our previous conclusion (Gamaley et al., 2006). Decrease in 3T3-SV40 sensitivity to NK cells activity
accompanied by actin reorganization resulted from both latrunculin B and NAC action suggests changes in cellular surface, which ultimately lead to inactivation
(or loss) of the molecules being activating signals to NK cells.
Key words: actin cytoskeleton, cytotoxicity index, latrunculin B, N-acetylcysteine, natural killers, transformed fibroblasts 3T3-SV40
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