ALLOCATION AND IDENTIFICATION OF FIBROCYTES FROM HUMAN PERIPHERAL BLOOD
E. V. Skorobogataya,1 N. V. Kalmykova, M. I. Blinova, G. P. Pinaev
Institute of Cytology RAS, St. Petersburg;
1 e-mail: rev22@mail333.com
Fibrocytes are the cells circulating in peripheral blood that synthesize a big number of various factors and
take part in the start of reclaiming processes. The wound healing is a result of activity of fibrocytes. It is known
that they participate in formation of hypertrophic and kelloid scars. The purpose of the present work was to research
specific properties. of fibrocytes in vitro. The data obtained testify that these cells really have hematopoietic
origin and are undifferentiated. In this connection, while cultivating fibrocytes it is necessary to keep to some
specific conditions: the use of the medium specific for stem cells and very high density of cultivation. In 10 days
of culturing, the fibrocytes differentiate into fibroblasts. From the general pool of peripheral blood mononuclear
cells, only fibrocytes are capable of DNA synthesis but in spite of it proliferative potential of these cells is very
low.
Key words: peripheral blood, fibrocytes, wound healing
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