ABOUT THE CHARACTERS OF THERMOPROTECTION IN INFLUENCE OF OSMOLITES ON BACTERIA
I. I. Morozov, V. G. Petin
Medical Radiological Research Center RAMS, Obninsk;
e-mail: mrrc@obninsk.ru
The influence of the length of staying of Escherichia coli B/r cells in hypertonic NaCl solution before heating
at 52 and 60 °C on the magnitude of salt thermoprotection was investigated. In addition, the dependence of
the isotonic and thermoprotective NaCl concentrations on the exposure temperature was investigated. It was
shown that the volume of cell osmotic thermoprotection was independent on the length of preliminary staying of
microorganisms in hypertonic NaCl solution. It was also shown that the magnitude of isotonic and thermoprotective
osmolite concentrations increased with the increase in the exposure temperature. The analysis of the data
obtained and published in literature indicates that the compensating mechanism is involved in salt bacteria thermoprotection
rather than the dehydratation one.
Key words: heating, salt thermoprotection, dehydratation, osmotic homeostasis, bacteria survival
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