THE DENSITY OF MYOCARDIAL AND PERICARDIAL RAT MAST CELLS IN ISOPROTERENOL-INDUCED HEART FAILURE
I. L. Erokhina,1 S. V. Okovityy,2 A. N. Kulikov,2 A. A. Kazachenko,2
M. G. Martynova,1 O. M. Moiseeva,3 S. N. Shulenin,2 O. I. Emelyanova 1
1 Institute of Cytology RAS, 2 Military Medical Academy and
3 V. A. Almazov Research Institute of Cardiology, St. Petersburg;
1 e-mail: heartdev@mail.cytspb.rssi.ru
Myocardial mast cells (MC) respond to cardiovascular pathology. The behavior of MC population in myocardium
and pericardium of rats has been studied 24 h, 14, 28 and 60 days after two isoproterenol injections (at
24 h intervals). The extent of heart failure has been estimated by supersonic inspection 28 and 60 days after
isoproterenol injections. The density of MCs of different degrees of maturity was estimated on paraffin sections
stained with Alcian blue-Safranin. The MC density in myocardium of intact and experimental rats was relatively
low: from 4 to 6 cells/mm2. The MC density in pericardium of intact rats was several times higher than in
myocardium: 48.6 ± 13.0 cells/mm2. In 24 h and 14 days after isoproterenol injections the pericardial MC density
was 1.5 times higher than in control rats (P < 0.05) at the expense of increase in the number of mature MCs
with Safranine-positive granules without the increase in the number of immature cells with Alcian blue-positive
granules. In 28 days the pericardial MC density was 2 times higher than in intact rats (P < 0.05) at the expense
of increase in number of immature and mature cells. In 60 days after isoproterenol injections the pericardial MC
density and the ratio of immature and mature cells compared with control did not reach statistical significance.
The changes in pericardial MC population corresponded to severity of heart failure according to functional indices.
The findings show active reaction of pericardial MCs on myocardium dysfunction that stimulates the maturation
of resident immature MCs in pericardium and migration of immature cells to pericardium of damage heart.
Key words: mast cells, myocardium, pericardium, heart failure, isoproterenol, rats
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