HIGH TEMPERATURE INITIATES CHANGES OF WOLBACHIA ULTRASTRUCTURE IN THE OVARIES AND EARLY EMBRYOS OF
DROSOPHILA MELANOGASTER
M. V. Zhukova, D. A. Voronin, E. V. Kiseleva 1
Institute of Cytology and Genetics, Siberian Branch of RAS, Novosibirsk;
1 e-mail: elka@bionet.nsc.ru
Electron microscopic analysis of Drosophila melanogaster (w1118) ovarian cells has demonstrated that stressful heat treatment of flies results in the
appearance of electron dense granules and large lysosomes in the cytoplasm of ovarian cells, which is not related with the presence of Wolbachia, as these
changes are observed in both the infected and uninfected flies. High temperature initiates essential envelope defects and other structural changes of symbiotic
bacteria in the cytoplasm of ovarian cells. Some embryos developing from eggs of heat shocked flies die, however, bacteria in the survival embryos retain their
typical morphology. Endosymbionts do not change their localization and their contacts with the mitochondria and endoplasmic reticulum in the ovarian cells and
early embryos after heat shock treatment of the flies. The results obtained show that high temperature influences on both the host and the endosymbiont, but does
not change their structural mutual interactions.
Key words: heat shock, Wolbachia, Drosophila melanogaster, ovaries, embryos, endosymbiotic bacteria, ultrastructure
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