CHARACTERIZATION OF CULTURED MURINE MESENCHYMAL STEM CELL LINE EXPRESSING GFP
T. M. Grinchuk,1 K. M. Ivantsov,1 L. L. Alekseenko,1 I. V. Kozhukharova,1
A. M. Zaichik,2 N. S. Petrov,1 V. M. Mikhailov,1 B. V. Popov 1
1 Institute of Cytology RAS, St. Petersburg, and 2 St. Petersburg State Medical Academy of Postgraduate Education;
e-mail: gmdmk@mail.cytspb.rssi.ru
We have developed and characterized a murine mesenchymal stem cell line from the bone marrow of transgenic mouse C57BL ubiquitously expressing GFP.
Immunostaining analysis revealed the presence of several markers typically found in fibroblasts such as smooth muscle cells actin in the form of stress fibrils and
vimentin - the protein of intermediate filaments. These cells maintained capability to differentiate into adipocytes or osteoblasts under appropriate conditions.
Karyotypic features include changes in the ploidy level between 2n and 8n and multiple chromosomal aberrations. After six passages 80 % of the cell population
was aneuploid with chromosomal numbers between 50 and 85 without well defined modal class. Differential G-staining of metaphase spreads showed variability in
copy numbers of individual chromosomes and the presence of aberrations such as ectopic associations of non-homologous chromosomes. All analyzed cells
contained unique dicentric marker chromosome and some of them also had numerous microchromosomes which might indicate gene amplification. These cells
could be useful in the future work directed at the investigation of stem cells spontaneous oncogenic transformation in vivo and in vitro.
Key words: mesenchymal stem cells, chromosomes, karyotypic destabilization, cell differentiation, green fluorescence protein, transgene mouse
Back
Contents
Main
|
