OSTEOGENIC DIFFERENTIATION OF HUMAN MULTIPOTENT MESENCHYMAL STROMAL CELLS OF BONE MARROW AND ADIPOSE TISSUE
I. P. Savchenkova,1 M. S. Rostovskaya,2 N. I. Chupikova,2 S. Z. Sharifullina,2
A. S. Teplyashin 2
1 Ya. R. Kovalenko All-Russian State Research Institute of Experimental Veterinary RAAS
and 2 Institute of Stem Cell, Moscow;
1 e-mail: S-IP@mail.ru@mail.ru
Cellular populations with phenotype similar to multipotent mesenchymal stromal cells have been isolated from two different sources: a human bone marrow and
adipose tissue. The comparative analysis of differentiation efficiency of these cells in the direction of osteogenesis revealed morphological changes confirmed by
Alizarin red and von Kossa staining in bone marrow cells on the 14th day, and in adipose tissue cells on the 28th day of culturing in the
medium with inductors. The analysis of osteopontin, osteocalcin and bone sialoprotein gene expression in RT-PCR reactions detected essential
distinctions in the potency of these cells to differentiate into bone tissue cells.
Key words: multipotent mesenchymal stromal cells, bone marrow, adipose tissue, differentiation, osteogenesis, in vitro, gene expression
Back
Contents
Main
