EFFECTS OF PLASMINOGEN, STREPTOKINASE AND THEIR EQUIMOLAR COMPLEXES WITH PYRUVATE KINASE ON THE HUMAN
NEUROBLASTOMA IMR-32 CELLS
A. A. Romanovskaya,1 V. N. Nikandrov
Institute of Physiology, National Academy of Sciences of Belarus, Minsk;
1 e-mail: a-a-r@tut.by
The system of extracellular proteolysing, consists of plasminogen (PGn), its active protease (plasmin), PGn
activation and PGn activators inhibitors, influences the nervous tissue functions, their growth, differentiation and
proliferation in both, normal and pathological conditions. The purpose of the investigation was to study the effects
of exogenous PGn, its activator streptokinase (SK), PK and their equimolar complex on the morpho-functional state
neuroblastoma IMR-32 cells. PGn, SK, PK and their complexes stimulated cells proliferation during 1-3 days of
incubation, shown by cell quantity increase. We also observed DNA, RNA and protein increase. The low lactate
dehydrogenase efflux was evidence of that an addition of the proteins under investigation in the culture medium
prevented the development of degenerative alterations connected with serum deprivation. The levels of extracellular
PGn-activator activity, as measured by the biochemical fibrinolytic assay, increased over SK. This SK effect vanished
on the 3rd day when SK formed complexes with PK. New original facts obtained testify the probability of initiation of
neoplastic transformation and tumor growth potentiation.
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