MESENCHYMAL STEM CELLS-BASED THERAPY OF BRAIN ISCHEMIC STROKE IN RAT
N. N. Zinkova,1, * E. G. Gilerovitch,2 I. B. Sokolova,1
S. K. Vijde,1 E. V. Shvedova,1 G. V. Alexandrov,1 P. V. Kruglyakov,1
Kislyakova T. V.,1 D. G. Polyntsev 1
1 "Trans-Technologies", Ltd., 1 State Institute of Experimental Medicine RAS,
St. Petersburg;
Mesenchymal stem cells (MSCs) - based therapy is a promising modern attempt to improve the recovery after stroke.
Experiments were carried out on inbred Wistar-Kyoto rats. MSCs were isolated, expanded in cultute and labeled with
vital fluorescent dye PKH-26. Animals were subjected to middle cerebral artery occlusion (MCAO), followed by injection
of 5·106 rat MSCs into the tail vein 3 days after MCAO. Control group animals received PBS injection
(negative control). Therapy results were estimated by the following parameters: behavioral and neurological testing,
the brain injure area, the state of damaged region "border" zone and the vessels quantity in the "border" area. It
was shown that control group animals (PBS injection) did not restore their initial behavioral and neurological state,
while the experimental group animals (MSCs injection) showed the same parameters as intact rats at 2-3 weeks after
MCAO. The size of the damaged region in the control group was approximately 1.5 as large as in the experimental group.
The damage in the experimental group was limited to neocortex; caudate nucleus, capsula externa and piriform cortex
remained uninjured. Small vessels quantity in the "border" regions was twine higher compared to control group and was
approximately equal to an intact brain vessel number. Moreover, it was shown for the first time that after MSCs
transplantation the vessels quantity in the neocortex and caudate putamen of contralateral hemisphere was twice as
much as in control. We demonstrated that the MSCs transplantation definitely exerted a positive influence upon the
brain tissue reparation after stroke.
Key words: mesenchymal stem cells, stroke, capillaries. size of the brain damage region
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