SC35 SPLICING FACTOR AND COILIN ARE COLOCALIZED WITHIN THE "ENDOBODIES"
IN OOCYTES OF THE SPIDER ARANEUS DIADEMATUS
D. S. Bogolyubov,1 I. O. Bogolyubova
Institute of Cytology RAS, St. Petersburg;
1 e-mail: dmitr@mail.cytspb.rssi.ru
Oocytes of the spider Araneus diadematus are solitary developed. The oocyte nuclei are transcriptionally
active, as revealed using microinjections of 5-bromouridine 5'-triphosphate into the ooplasm. The nucleus contains
several extrachromosomal structures of perfectly spherical shape, the so-called endobodies. Laser scanning
confocal microscopy revealed intense fluorescence of endobodies when an antibody against the splicing factor
SC35, a major component of interchromatin granule clusters (IGCs), was applied. At the same time, colocalization
of SC35 protein with coilin, a marker protein of Cajal bodies (CBs), was also observed within these structure.
We suggest that endobodies of A. diadematus oocytes may have some features of both IGCs and CBs and represent
a single nuclear domain.
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