CHROMOSOMAL REARRANGEMENTS AND THEIR EFFECTS IN SPONTANEOUS IMMORTALIZATION AND TRANSFORMATION OF RAT EMBRYO CELLS
IN VITRO
N. M. Yartseva,1 R. F. Fedortseva,2 I. V. Artsybasheva 1
1 Institute of Cytology RAS, and 2 All-Russian Centre of Emergency and Radiation Medicine,
Emercom of Russia, St. Petersburg;
1 e-mail: Yartseva@mail.cytspb.rssi.ru
G-banding analysis of LRec-1 and LRec-3, spontaneously immortalized cell lines from rat embryo fibroblast,
revealed diploid karyotypes with specific clonal structural rearrangements of chromosomes 7 and 19 -
del(7)(q11.2q22.1), t(7;19)(q11.1;q12) in malignant stage. Both clonal abnormalities of chromosomes 7 and 19
were also revealed in LRec-1 k clone and LRec-1 sf cell line. Previous study of LRec-1 and LRec-3 cells showed
the presence of karyotypes with pseudodiploid modal chromosome number, partial trisomy of chromosome 7 and
same clonal structural rearrangements of chromosomes 7 and 19 in immortalized stage. In malignant stage, the trisomy
6 and new clonal structural rearrangements of chromosomes 1, 2, 11, 15, 18, 19 and of chromosomes 10, 20
were also found in LRec-1 sf and LRec-1 cells, accordingly. There were no new clonal structural chromosome rearrangements
in LRec-1 k and LRec-3 cells. We compared locies of chromosomes involved in rearrangements
with mapped genes on these chromosomes according to RATMAP. Supposedly these genes are involved in spontaneous
immortalization of rat embryo fibroblast and malignant transformation of LRec-1 and LRec-3 cells and
rearrangements of chromosomes 1, 2, 11, 15 and 18 facilitate expression of growth factors of LRec-1 sf cells.
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