HEAT SHOCK PROTEIN OF 70 kDa IN PARAMECIUM NEPHRIDIATUM AND ITS ROLE IN ADAPTATION TO ENVIRONMENTAL SALINITY
CHANGES
A. O. Smurov,1 Yu. I. Podlipaeva,2 A. V. Goodkov 2, *
1 Zoological Institute RAS, and 2 Institute of Cytology RAS, St. Petersburg;
* e-mail: ogalibin@mail.ru
The level of Hsp70 was studied in the cells of eurihaline ciliate Paramecium nephridiatum after the environmental
salinity changes. Two types of treatment were applied. "Shock": ciliates were placed for 1 h to the
medium with stress salinity, then transferred back to the medium, they were acclimated to, for 2 h; "adaptation":
ciliates were placed for 3 h into stress salinity. It has been shown, that ciliates, acclimated to fresh water (0 ‰)
have the higher constitutive level of Hsp70, than those, acclimated to 10 ‰. Transfer from fresh water to 10 ‰
does not cause the increase of Hsp70 synthesis in protists, whereas the reciprocal transfer results in induction of
Hsp70 in the cells. "Adaptation" results in induction of Hsp70 in both "directions" of salinity changes. The results
obtained allow to presume that the possibility to survive in the media of various salinity in eurihaline ciliates
is somehow determined by the higher initial level of Hsp70 in their cells, than in stenohaline representatives
of the same genus.
Key words: salinity adaptation, freshwater ciliates, eurihaline ciliates, Paramecium nephridiatum,
Paramecium jenningsi, heat shock proteins
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