INFLUENCE OF AUTOCRINE FACTOR DEFICIT IN CULTURE ON SURVIVAL AND ENERGY METABOLISM OF CTLL-2 CELLS UNDER OXIDATIVE
STRESS
G. V. Lutsenko, M. V. Grechikhina, L. G. Diachkova, N. I. Lutsan
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry RAS, Moscow;
* e-mail: ogalibin@mail.ru
A lot of data has shown recently that survival of mammalian cells is under a control of growth factors and
autocrine survival factors (AF). We studied the influence of AF deficit on survival, intracellular ATP content,
and transmembrane potential of mitochondria of IL-2-dependent CTLL-2 cells under oxidative stress. CTLL-2
cells cultivated under deficit of AF have been shown to be more susceptible to oxidative injury in comparison
with the cells cultivated without deficit of AF (control); they died at smaller concentrations of H2O2 than control
cells did. The ATP content in CTLL-2 cells was decreased under AF deficit conditions even without any stress
and treatment of the cells by hydrogen peroxide resulted in additional large decrease of it. ATP depression was
accompanied by disruption of cell membrane (blebbing) and drop of mitochondrial potential. Cell death under
oxidative stress in the presence of AF deficit has been shown to proceed by both apoptosis and necrosis.
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