RETROSPECTIVE MOLECULAR-CYTOGENETIC CHARACTERISTICS OF TETRAPLOIDY IN EARLY EMBRYOLETHALITY OF MAN
A. A. Kashevarova,1 N. N. Sukhanova, E. N. Tolmacheva, E. A. Sazhenova, I. N. Lebedev
Research Institute of Medical Genetics, Tomsk Research Center of Siberian Branch RAMS, Tomsk;
1 e-mail: kashevarova_a@rambler.ru
The ploidy level of noncultivated extraembryonic tissues was studied by fluorescence in situ hybridization
in 30 human I trimester spontaneous abortions with tetraploid or diploid-tetraploid karyotype after conventional
cytogenetic analysis. Only thirteen embryos (43 %) were verified to be tetraploid that provides evidence for the
hypothesis of placental cell polyploidization during long-term in vitro cultivation. It is shown that preferred
compartmentalization of tetraploid cells in the inner cell mass derivatives is associated with blighted ovum -
the most severe type of human embryo dysmorphogenesis.
Key words: tetraploidy, long-term cell cultures, spontaneous abortions, interphase cytogenetics
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