THE PECULIARITIES OF ELECTROSTATIC INTERACTION OF PANCREATIC AND MICROBIAL RIBONUCLEASES WITH MACROPHAGES
IN VITRO
N. V. Kalacheva, A. V. Narulina, B. M. Kurinenko
Department of Microbiology, Laboratory of Engineering Enzymology, Kazan State University;
e-mail: Natalia.Kalacheva@ksu.ru
The interaction of RNase A and RNase Bacillus intermedius with peritoneal macrophage of rats has been
studied. To estimate the efficiency of this interaction the spontaneous chemiluminescence and induced by phagocytosis
chemiluminescence of macrophages were investigated. It has been shown that electrostatic interaction
of enzyme proteins with negatively charged cell membrane makes substantional contribution to the development
of chemiluminescence reply of macrophages. The RNase A, which is more basical than the other (i. e. it has larger
value of pI) is less effective with respect to macrophages. The calculation of total charge and dipole moment
of pancreatic and microbial RNases showed that the efficiency of interaction between protein polycation and a
cell was not connected with pI and depended on the charge and its distribution on the surface of protein molecule
at the given pH value.
Key words: RNase A, RNase Bacillus intermedius, macrophage, electrostatic interaction, total
charge and dipole moment
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