THE INFLUENCE OF IMMOBILIZED FIBRONECTIN ON KARYOTYPIC VARIABILITY OF TWO RAT KANGAROO KIDNEY CELL LINES
G. G. Poljanskaya, T. S. Goryachaya, G. P. Pinaev
Institute of Cytology RAS, St. Petersburg;
e-mail: poljansk@mail.cytspb.rssi.ru
The numerical and structural karyotypic variability has been investigated in "markerless" Rat kangaroo kidney
cell lines NBL-3-17 and NBL-3-11 when cultivating on a fibronectin-coated surface. In cell line NBL-3-17, cultivated
on the fibronectin-coated surface for 1, 2, 4 and 8 days, the character of cell distribution for the chromosome
number has changed. These changes involve a significant decrease in frequency of cells with modal number of
chromosomes, and an increase in frequency of cells with lower chromosomal number. Many new additional structural
variants of the karyotype (SVK) appear. The observed alterations seem to be due preference adhesion of cells with
lower chromosome number, disturbances of mitotic apparatus and selection of SVK, which are more adopted to changes
in culture conditions. Detachment of cells from the fibronectin-coated surface, followed by 5 days cultivation on a
hydrophilic surface restored control distribution. In cell line NBL-3-11, cultivated on the fibronectin-coated
surface for 1, 2, 4 and 8 days, the character of numerical karyotypic variability did not change compared to control
variants. In cell line NBL-3-17 the frequency of chromosomal aberrations under cultivation on the fibronectin-coated
surface for 1, 2, 4 and 8 days did not change relative to control variants. In cell line NBL-3-11 the frequency of
chromosomal aberrations under the same conditions significantly increases, mainly at the expence of chromosomal,
chromatid breaks and dicentrics (telomeric association) relative to control variants. We discuss possible reasons of
differences in the character of numerical and structural karyotypic variability between cell lines NBL-3-17
(hypotriploid) and NBL-3-11 (hypodiploid) under cultivation on fibronectin. The reasons of the observed interline
karyotypic differences possibly consist in peculiarity of karyotypic structure of cell line NBL-3-11 and in the
change of gene expression, namely in a dose of certain functioning genes in the hypotryploid cell line NBL-3-17.
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