CYTOGENETIC AND IMMUNOLOGICAL SPECIFICITY OF MAMMALIAN HYBRID CELL LINES
N. M. Grezina,1 P. M. Klenovitskiy, N. A. Zinovieva, E. A. Gladyr, Yu. V. Konoplenko,
L. K. Ernst
All-Russian State Research Institute of Animal Breeding of the Russian Academy of Agricultural Science,
Dubrovitsy Settlement, Podolsk District, Moskow Region;
1 e-mail: N-Grezina@yandex.ru
Karyotypes of the hybrid cell lines NS-RL-3 (าส--sheep kidney cells and rabbit lymphocytes) and
βCR-NS (าส--rabbit β-cells and าส--sheep kidney cells) were investigated. It was
shown that both hybrid cell lines were characterized by presence of both sheep and rabbit chromosomes, which number
and structure varies depending on the cell type and the number of passages. In some cases the aberrant chromosomes
were identified. It was observed, that 40-50 % of the NS-RL-3 cells survived in culture in the presence of the human
blood serum, and also were identified during 7-28 days after their introduction into the organism of the animal.
Thus, the partial immunological tolerance of the hybrid cell lines has been suggested.
Key words: hybrid cells, karyotype, a barrier histouncompatibility
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