ESTIMATION OF CELL VIABILITY USING THEIR GEOMETRICAL PARAMETERS BY THE EXAMPLE OF CULTIVATED FIBROBLASTS
I. G. Gerasimov,1 A. G. Popandopulo 2
1 Research Institute of Family Medical Problems of Donetsk State Medical University, and
2 Research Institute of Urgent and Reconstractin Surgery, Donetsk, Ukraine;
e-mail: IGGerasim@mail.ru
The viability of cultivated fetal fibroblasts has been estimate cytochemically, by system of expert estimations
and by entropy equivalent (EE) generate, on the basis of the thermodynamic theory of nonequlibrium processes. The EE
is the superposition of the transformed correlation matrix elements which are calculated by geometrical parameters
of cell projections, using perimeter, the area, the compactness, and the central moments of the first and third
orders, their difference and Fourier Description. Geometrical parameters of cells, which are the most effective for
the estimation of viability by EE, and also necessary and sufficient for such estimation of cell number, are
determined. It was shown, that cytochemical and expert estimations of viability well correlate with value of EE,
which, according to thermodynamic representations, grows during artificial destruction of culture and at
deterioration of its quality.
Key words: fetal fibroblasts, cell viability, cell size, cell form, entropy equivalent, expert
estimation, cytochemistry
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