DECOMPACTIZATION OF CHROMOSOME 1 IN THE ARTIFICIALLY DECONDENSED HUMAN SPERM NUCLEI: OVERALL TOPOLOGY AND
NON-RANDOM LOCATION OF CHROMOSOME BENDS
O. S. Mudrak,1,* N. V. Tomilin,1 A. O. Zalensky 2
1 Institute of Cytology RAS, St. Petersburg, and
2 Jones Institute for Reproductive Medicine, Eastern Virginia Medical School, USA;
* e-mail: o_mudrak@list.ru
We have used FISH with microdissected chromosome arm-specific DNA probes that allow direct visualization of
chromosome 1 folding in human sperm nuclei. We described the overall topology and the mode of packaging of sperm
chromosome 1 and thus established new elements of ordered genome architecture in these specialized cells.
Key words: sperm, nucleus, in situ hybridization, chromosome architecture
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